Transformation of the symbiotic alga Oophila amblystomatis: a new tool for animal-algae symbiosis studies

The ability to conduct reverse genetic studies in symbiotic systems is enabled by transgene expression and transformation of at least one partner. relationship between the yellow spotted salamander, Ambystoma maculatum, green alga, Oophila amblystomatis, a unique model vertebrate-algae symbiosis. Despite over 130 years scientific study, there are still many open questions this Transgene partner will accelerate research into relationship. In paper we describe tool method for foreign DNA in, presumed of, alga O. amblystomatis. We successfully introduced heritable antibiotic resistance algal cultures, observed fluorescent reporter protein all transfected presumably transformed populations. outcomes work enable manipulation allowing direct testing hypotheses derived from gene or genomic that usher deeper understanding A. maculatum-O. amblystomatis system.